In computer processor the negative flag or sign flag is a single bit in a system status (flag) register used to indicate whether the result of last mathematic operation resulted in a value whose most significant bit was set. In a two's complement interpretation of the result, the negative flag is set if the result was negative.
For example, in an 8-bit signed number system, -37 will be represented as 1101 1011 in binary (the most significant bit is 1), while +37 will be represented as 0010 0101 (the most significant bit is 0).
The negative flag is changed in the x86 series processors by the following instructions (referring to the Intel 80386 manual [1]):